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The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications Describes the angiogenic tree the mother’s uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success
This fourth edition of "The Mouse Manual¨is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on: · generation of induced pluripotent stem cells · RNA microinjections · lentiviral microinjections and infection · assisted reproduction techniques for sperm and embryo cryopreservation · isolation, generation, and transplantation of spermatogonial stem cell lines · in utero electroporation of gene constructs into postimplantation embryos · vibratome sectioning of live and fixed tissues for imaging thick tissue sections · whole-mount fluorescent staining methods for three-dimensional visualization Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.
Donor 3319 Profile: Tall. Blonde. Blue eyes. Medical Student. Wanted for Serial Murder. “Spellbinding. Another tour de force from Scottoline. It drew me in, in a single breath.” –Mary Kubica, bestselling author of The Good Girl Christine Nilsson and her husband, Marcus, are desperate for a baby. Unable to conceive, they find themselves facing a difficult choice they had never anticipated. After many appointments with specialists, endless research, and countless conversations, they make the decision to use a donor. Two months pass, and Christine is happily pregnant. But one day, she is shocked to see a young blond man on the TV news being arrested for a series of brutal murders—and the blond man bears an undeniable and uncanny resemblance to her donor. Delving deeper to uncover the truth, Christine must confront a terrifying reality and face her worst fears. Riveting and fast-paced with the depth of emotionality that has garnered Lisa Scottoline legions of fans, Most Wanted poses an ethical and moral dilemma: What would you do if the biological father of your unborn child was a serial killer?
An undocumented immigrant’s journey from a New York City homeless shelter to the top of his Princeton class Dan-el Padilla Peralta has lived the American dream. As a boy, he came here legally with his family. Together they left Santo Domingo behind, but life in New York City was harder than they imagined. Their visas lapsed, and Dan-el’s father returned home. But Dan-el’s courageous mother was determined to make a better life for her bright sons. Without papers, she faced tremendous obstacles. While Dan-el was only in grade school, the family joined the ranks of the city’s homeless. Dan-el, his mother, and brother lived in a downtown shelter where Dan-el’s only refuge was the meager library. There he met Jeff, a young volunteer from a wealthy family. Jeff was immediately struck by Dan-el’s passion for books and learning. With Jeff’s help, Dan-el was accepted on scholarship to Collegiate, the oldest private school in the country. There, Dan-el thrived. Throughout his youth, Dan-el navigated these two worlds: the rough streets of East Harlem, where he lived with his brother and his mother and tried to make friends, and the ultra-elite halls of a Manhattan private school, where he could immerse himself in a world of books and where he soon rose to the top of his class. From Collegiate, Dan-el went to Princeton, where he thrived, and where he made the momentous decision to come out as an undocumented student in a Wall Street Journal profile a few months before he gave the salutatorian’s traditional address in Latin at his commencement. Undocumented is a classic story of the triumph of the human spirit. It also is the perfect cri de coeur for the debate on comprehensive immigration reform. Praise for Undocumented “Dan-el Padilla Peralta’s story is as compulsively readable as a novel, an all-American tall tale that just happens to be true. From homeless shelter to Princeton, Oxford, and Stanford, through the grace not only of his own hard work but his mother’s discipline and care, he documents the America we should still aspire to be.” —Dr. Anne-Marie Slaughter, President of the New America Foundation
Everything you believe about how to lose weight is wrong. Weight gain and obesity are driven by hormones—in everyone—and only by understanding the effects of insulin and insulin resistance can we achieve lasting weight loss. In this highly readable and provocative book, Dr. Jason Fung sets out an original, robust theory of obesity that provides startling insights into proper nutrition. In addition to his five basic steps, a set of lifelong habits that will improve your health and control your insulin levels, Dr. Fung explains how to use intermittent fasting to break the cycle of insulin resistance and reach a healthy weight—for good.
This text provides an introduction to supercritical fluids with easy-to-use Excel spreadsheets suitable for both specialized-discipline (chemistry or chemical engineering student) and mixed-discipline (engineering/economic student) classes. Each chapter contains worked examples, tip boxes and end-of-the-chapter problems and projects. Part I covers web-based chemical information resources, applications and simplified theory presented in a way that allows students of all disciplines to delve into the properties of supercritical fluids and to design energy, extraction and materials formation systems for real-world processes that use supercritical water or supercritical carbon dioxide. Part II takes a practical approach and addresses the thermodynamic framework, equations of state, fluid phase equilibria, heat and mass transfer, chemical equilibria and reaction kinetics of supercritical fluids. Spreadsheets are arranged as Visual Basic for Applications (VBA) functions and macros that are completely (source code) accessible for students who have interest in developing their own programs. Programming is not required to solve problems or to complete projects in the text. Property worksheets/spreadsheets that are easy to use in learning environments Worked examples with Excel VBA Worksheet functions allow users to design their own processes Fluid phase equilibria and chemical equilibria worksheets allow users to change conditions, study new solutes, co-solvents, chemical systems or reactions
The selfie photography of Kim Kardashian West, featuring many never-before-seen personal images from one of the most recognizable and iconic celebrities in the world. From her early beginnings as a wardrobe stylist, Kim Kardashian West has catapulted herself into becoming one of the most recognizable celebrities in Hollywood. Hailed by many (including Givenchy designer Riccardo Tisci) as the modern-day personification of Marilyn Monroe, Kim has become a true American icon. With her curvaceous style, successful reality TV show Keeping Up with the Kardashians, DASH clothing store, makeup and perfume lines, she has acquired a massive fan following in the multi-millions. Through social media (Instagram, Twitter, and Facebook), Kim connects with her fans on a daily basis, sharing details of her life with her selfie photography. Widely regarded as a trailblazer of the "selfie movement"—a modern-day self-portrait of the digital age—Kim has mastered the art of taking flattering and highly personal photos of oneself. For the first time in print, this book presents some of Kim's favorite selfies in one volume—from her favorite throwback images to current ultra-sexy glam shots—and provides readers with a behind-the-scenes look into this larger-than-life star.

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