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The second edition of the perfect companion for practical course work in enzymology. Now with an improved selection of enzymatic assays based on key metabolic reactions, this book provides the detailed background to the enzymatic reaction and the enzyme per se. Particular emphasis is placed on troubleshooting with the described methods, and, in addition, the new four-color layout throughout features detailed protocols highlighted for easy recognition. A companion website provides animations for all figures together with supplementary material, for deeper understanding of the partially abstract matter.
Fully updated and expanded-a solid foundation for understanding experimental enzymology. This practical, up-to-date survey is designed for a broad spectrum of biological and chemical scientists who are beginning to delve into modern enzymology. Enzymes, Second Edition explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. The book provides illustrative examples from the contemporary literature to guide the reader through concepts and data analysis procedures. Clear, well-written descriptions simplify the complex mathematical treatment of enzyme kinetic data, and numerous citations at the end of each chapter enable the reader to access the primary literature and more in-depth treatments of specific topics. This Second Edition of Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis features refined and expanded coverage of many concepts, while retaining the introductory nature of the book. Important new features include: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors and multiple substrate reactions Coverage of current practical applications to the study of enzymology Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research.
The only text providing practical guidelines to a wide range of topics important to the practicing enzymologist. It offers advice on how solvents, salts, substrates,and denaturants affect enzyme function and stability; developing a scheme for purifying an enzyme from different kinds of tissue; applying different techniques for fractionating proteins; characterizing proteins by determining molecular weight, isoelectric pH, amino acid composition and sequence, ligand binding, and enzyme kinetics. Basic guidelines to procedures and hints for the operation of a technique are also included.
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Key Features * Provides general guidelines and strategies for isolation of membrane proteins * Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs * Gives special emphasis to new native and denaturing electrophoresis techniques * Explains modifications of techniques used for water-soluble proteins
Exceptionally clear coverage of mechanisms for catalysis, forces in aqueous solution, carbonyl- and acyl-group reactions, practical kinetics, more.
Corporate Governance is a comprehensive textbook specially designed to meet the requirements of postgraduate management students. It provides an in-depth analysis of the core concepts and supplements them with relevant examples, exhibits and case studies.The book begins with an exhaustive introductory chapter defining corporate governance and a director's role in a company. Further, divided into four parts, part 1 covers types of corporations, theories and models of governance. It also examines the events which have led to the current thrust ofcorporate governance addressing the Cadbury report in UK, Sarbanes-Oxley Act, 2002 in USA as well as the committees constituted by SEBI. Part 2 elaborates on the structure of the board and its development process. It also highlights the issues related to compensation, performance review of theDirector as well as the importance of leveraging good governance for competitive advantage. Part 3 gives a broad view of governance related problems associated with typical ownership patterns, and capital market institutions from a stakeholder's perspective. The ground rules for a director toperform his responsibilities and duties are also discussed in this part. The book concludes with a chapter discussing the future of corporate governance in part 4.Owing to its comprehensive coverage and approach, students will find this book useful. It will also serve as a veritable guide for professionals owing to the inclusion of varied examples and case studies.

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